EXPERIMENTAL DETAILS
Materials RequiredCurcurm Longa Tubers (from local super market), Cerium Nitrate (Finar chemicals,India Pvt. Ltd), CeriumNitrate (Sigma Rich), Acetone, Ethanol, and Distilled Water were used in the synthesis process intheir pure form without any further modification.
Extraction of Curcuma longa tubers powder
The C. longa tubers were purchased from a local market; The C. longa tubers were washed to remove the adhering mud particles and possible impurities. Later they were dried under sunlight for a week to completely remove the moisture. The tubers were cut into small pieces, powdered in a mixer, and then sieved using a 20-mesh sieve to get uniform size range. The final sieved powder was used for all further studies. For the production of extract, 0.3 g of C. longa tubers powder was added to a 200 mL flask with 100 mL Ethanol and then mixed for 4 hours at 70 oc temperature.
Biological Synthesis of CeO2 nanoparticles
In this Method 0.5 M of Cerium Nitrate was added to water under Constant Stirring. The Curcuma longa tubers powder Extraction was introduced to above solution in different sets (5, 10, 15, 20 and 25 ml), this was then placed in a Magnetic stirrer for 2 hrs at 70oC temperature. The resultant solution was placed in Autoclave for 5 Cycles (1cycle = 20 mins). After terminating the reaction, Semi liquid yellow precipitation obtained which was placed in Microwave until the evaporation of solvent. After terminating the reaction, the pale white precipitate was then taken out and washed over and over again with distilled water followed by ethanol to get free of the impurities and then CeO2 nanoparticles was obtained.